INDUSTRY WORKSHOP: Quantitative and simultaneous plasma drug analysis with LC-MS/MS for therapeutic drug monitoring
Tracks
Track 2
| Wednesday, September 24, 2025 |
| 12:30 PM - 1:25 PM |
| Grand Copthorne Waterfront Hotel - Waterfront Ballroom I |
| Sponsored By: |
Overview
Delivered by: Shimadzu Asia Pacific
Details
Therapeutic drug monitoring (TDM) is an important approach in personalized medicine. Recently, liquid chromatography/tandem mass spectrometry (LC-MS/MS) methods have come into clinical use including TDM. However, the introduction of TDM with LC-MS/MS has presented several challenges. One is method development in each laboratory.
Another issue is that LC-MS/MS requires pretreatment processing techniques and time. Furthermore, preparation of optimal MS/MS instrument in your facility is problematic.
Therefore, in this seminar, we would like to show the basics of drug plasma LC-MS/MS analysis method development, automation pretreatment processing for LC/MS/MS, and the possibility of using a time-of
flight MS/MS equipment for TDM. For plasma drug analysis, triple quadrupole MS/MS or quadrupole-time-of-flight hybrid MS/MS were used.
After the addition of internal standard, samples were pretreated with acetonitrile for protein precipitation with a fully automated sample preparation module.
The developed method, which was verified by method validation, was used to analyze drug plasma concentrations of clinical drugs. For antipsychotics, anticancer drugs, immunosuppressants, and antifungal drugs, the simultaneous drug plasma concentration analysis methods were developed successfully by LC-MS/MS and applied to clinical practice and research for TDM.
There was no difference in plasma drug concentrations between automated pretreatment processing and human procedure. QTOF MS/MS might be also applied for quantitative plasma drug analysis. LC-MS/MS is useful for drug analysis for clinical TDM practice, and it is evolving to be more easily implemented in clinical TDM.
Speaker
A/Prof Masamitsu Maekawa
Department of Pharmaceutical Sciences, Tohoku University Hospital
Presenter
Biography
Associate Professor Masamitsu Maekawa is an Associate Professor and Deputy Director in the Department of Pharmaceutical Sciences at Tohoku University Hospital, specializing in Clinical and Analytical Chemistry. He earned his BSc, MSc, and PhD from Tohoku University. Beginning his career as a pharmacist in 2009, he later became his research from 2010. He has authored 86 original papers including most cited and downloaded award paper and 12 review papers using clinical mass spectrometry. His contributions have earned him 18 awards, including the Encouragement Awards from the various societies, such as The Pharmaceutical Society of Japan and Japan Society of Clinical Chemistry.
